Difference between revisions of "Part:BBa K4202045:Experience"
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<p>The sequence of this part was synthesis by Genscript and constructed to the pHY300PLK by In-Fusion clone.</p> | <p>The sequence of this part was synthesis by Genscript and constructed to the pHY300PLK by In-Fusion clone.</p> | ||
<b>Characterization</b> | <b>Characterization</b> | ||
| − | < | + | <p>The transformant (<i>Bacillus subtilis</i> with pHY300PLK empty plasmid and kill switch) were inoculate to 2ml LB medium and cultured in the test tube overnight. The next day, the culture were inoculated into 2ml TB medium containing 1% sucrose (w/v) at the ratio of 1:100. After the OD<sub>600nm</sub>(L=1mm) reaches around 0.1, the OD<sub>600nm</sub>(L=1mm) were measured every 40minutes.</p> |
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==Result== | ==Result== | ||
Revision as of 13:04, 12 October 2022
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
The characterization experiment
Method
Measurement device construction
The sequence of this part was synthesis by Genscript and constructed to the pHY300PLK by In-Fusion clone.
Characterization
The transformant (Bacillus subtilis with pHY300PLK empty plasmid and kill switch) were inoculate to 2ml LB medium and cultured in the test tube overnight. The next day, the culture were inoculated into 2ml TB medium containing 1% sucrose (w/v) at the ratio of 1:100. After the OD600nm(L=1mm) reaches around 0.1, the OD600nm(L=1mm) were measured every 40minutes.
Result
User Reviews
UNIQe51c6fb786e6a858-partinfo-00000000-QINU UNIQe51c6fb786e6a858-partinfo-00000001-QINU