Difference between revisions of "Part:BBa K4244002"
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A silent mutation was also performed to removed the SapI site present in <partinfo>BBa_K3143689</partinfo> at position 13 (A to G) to make it RFC[1000] compatible.
 
A silent mutation was also performed to removed the SapI site present in <partinfo>BBa_K3143689</partinfo> at position 13 (A to G) to make it RFC[1000] compatible.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 12:56, 12 October 2022


sfGFP without stop codon


The stop codon of sfGFP (BBa_K3143689) was removed to allow C-terminus fusion with signal peptide.

A silent mutation was also performed to removed the SapI site present in BBa_K3143689 at position 13 (A to G) to make it RFC[1000] compatible.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]