Difference between revisions of "Part:BBa K4341001:Experience"

Revision as of 12:53, 12 October 2022

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4341001

Experience by 2022 iGEM OhioState

We fused mCherry to BBa_K4341001 in an attempt to determine its expression profile. Once the fusion of mCherry and T5 was obtained we attempted to ligate into the pET28a backbone (given to us by Dr. David Wood's Lab). However once isolation via gel extraction was done for our backbone and inserts were digested with BamHI and NotI ligation transformations proved fruitless after many attempts and swapping of buffers. Eventually because of time constraints were unable to pursue the problem any further.

Figure 1. Gel Electrophoresis Result: Lane 1 rrnB P1; Lane 2 pR; Lane 3 pR-rrnB P1; Lane 4 t5-pR

User Reviews

UNIQ2178753811c3b567-partinfo-00000002-QINU UNIQ2178753811c3b567-partinfo-00000003-QINU

@@ Line 5: / Line 5: @@
 ===Applications of BBa_K4341001===
+== '''Experience by [https://2022.igem.wiki/ohiostate/ 2022 iGEM OhioState] ''' ==
+We fused mCherry to BBa_K4341001 in an attempt to determine its expression profile. Once the fusion of mCherry and T5  was obtained we attempted to ligate into the pET28a backbone (given to us by Dr. David Wood's Lab). However once isolation via gel extraction was done for our backbone and inserts were digested with BamHI and NotI ligation transformations proved fruitless after many attempts and swapping of buffers. Eventually because of time constraints were unable to pursue the problem any further.
+<center><html><img src='https://static.igem.wiki/teams/4341/wiki/engineering/gel.svg' width='480px'></html></center>
+<center><html>Figure 1. Gel Electrophoresis Result: Lane 1 rrnB P1; Lane 2 pR; Lane 3 pR-rrnB P1; Lane 4 t5-pR</html></center>
 ===User Reviews===