Difference between revisions of "Part:BBa K4438802"
| Line 2: | Line 2: | ||
<partinfo>BBa_K4438802 short</partinfo> | <partinfo>BBa_K4438802 short</partinfo> | ||
| − | MN4_Target_1(BBa_K4438802) is a single-stranded DNA sequence having 76 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. The 3’ end has ssDNA antisense T7 promoter sequence and the 5’ end has broccoli cDNA (antisense) sequence. | + | MN4_Target_1(<partinfo>BBa_K4438802</partinfo>) is a single-stranded DNA sequence having 76 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. The 3’ end has ssDNA antisense T7 promoter sequence and the 5’ end has broccoli cDNA (antisense) sequence. |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This part along with MN4_trigger_1-T7promoter-w/oPol(BBa_K4438801) and Aptamer_MN4(BBa_K4438800) can be used to detect different concentrations of quinine [1]. | + | This part along with MN4_trigger_1-T7promoter-w/oPol(<partinfo>BBa_K4438801</partinfo>) and Aptamer_MN4(<partinfo>BBa_K4438800</partinfo>) can be used to detect different concentrations of quinine [1]. |
| − | + | ||
| + | [[File:T--IISER-Tirupati_India--mn4structure1.png]] | ||
Latest revision as of 12:48, 12 October 2022
MN4_Target_1
MN4_Target_1(BBa_K4438802) is a single-stranded DNA sequence having 76 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. The 3’ end has ssDNA antisense T7 promoter sequence and the 5’ end has broccoli cDNA (antisense) sequence.
Usage and Biology
This part along with MN4_trigger_1-T7promoter-w/oPol(BBa_K4438801) and Aptamer_MN4(BBa_K4438800) can be used to detect different concentrations of quinine [1].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Reinstein, O., Yoo, M., Han, C., Palmo, T., Beckham, S. A., Wilce, M. C., & Johnson, P. E. (2013). Quinine binding by the cocaine-binding aptamer. Thermodynamic and hydrodynamic analysis of high-affinity binding of an off-target ligand. Biochemistry, 52(48), 8652-8662.