Difference between revisions of "Part:BBa K4367012:Design"

 
 
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===Design Notes===
 
===Design Notes===
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Effort was put in to fin a good length of the linker between dCas9 and nTEV. Also, effort was put in to finding good binding sites for dCas9 with gRNA, in relation to binding strength, off-target binding, and distance between the two bindingsites of dCas9 (The split halves of TEVp must reach each other).
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Effort was put in to find a good length for the linker between dCas9 and nTEV. Also, effort was put in to finding good binding sites for dCas9 with gRNA, in relation to binding strength, off-target binding, and distance between the two binding sites of dCas9 (The split halves of TEVp must reach each other).
  
  

Latest revision as of 12:43, 12 October 2022


dCas9-nTEV


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4636
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 14
    Illegal BsaI.rc site found at 4643


Design Notes

Effort was put in to find a good length for the linker between dCas9 and nTEV. Also, effort was put in to finding good binding sites for dCas9 with gRNA, in relation to binding strength, off-target binding, and distance between the two binding sites of dCas9 (The split halves of TEVp must reach each other).


Source

dCas9 is a variant of Cas9 from the CRISPR/Cas9 system. nTEV is a half of split TEVp, which is Tobacco Etch Virus Protease.

References