Difference between revisions of "Part:BBa K4298008:Design"
GrizzlyZhao (Talk | contribs) |
|||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | . | + | Cloned by PCR from pTac-RJN-AcrB-mCh with AcrB sequence deleted. |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
Latest revision as of 12:43, 12 October 2022
AcrB empV
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 3104
Illegal SpeI site found at 908 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SpeI site found at 908
Illegal NotI site found at 915 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BamHI site found at 2108
Illegal XhoI site found at 2143
Illegal XhoI site found at 4066 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 3104
Illegal SpeI site found at 908 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 3104
Illegal SpeI site found at 908
Illegal NgoMIV site found at 2259
Illegal AgeI site found at 2672 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 4141
Design Notes
Cloned by PCR from pTac-RJN-AcrB-mCh with AcrB sequence deleted.