Difference between revisions of "Part:BBa K4162001"

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===Worth to Notice===
 
===Worth to Notice===
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This part is an improved version of StayGold fluorescent reporter.
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This biobrick is the version of StayGold available on [https://www.addgene.org/186296/ Addgene].
  
 
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Revision as of 12:33, 12 October 2022


StayGold = (n2)oxStayGold(c4)v2.0


Introduction

2022 Fudan

A new version of StayGold[1], which gives a higher expression level than the original version, and is over one order of magnitude more photostable than EGFP. The StayGold gene has been codon optimization for exogenous expression.

Worth to Notice

This biobrick is the version of StayGold available on Addgene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 638
    Illegal BsaI.rc site found at 658


  1. ↑ A highly photostable and bright green fluorescent protein. Hirano M,  Ando R,  Shimozono S,  Sugiyama M,  Takeda N,  Kurokawa H,  Deguchi R,  Endo K,  Haga K,  Takai-Todaka R,  Inaura S,  Matsumura Y,  Hama H,  Okada Y,  Fujiwara T,  Morimoto T,  Katayama K,  Miyawaki A. Nat Biotechnol, 2022 Jul;40(7):1132-1142. PMID:35468954