Difference between revisions of "Part:BBa K4438602"
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<partinfo>BBa_K4438602 short</partinfo> | <partinfo>BBa_K4438602 short</partinfo> | ||
| − | T6_Target_1 is a single-stranded DNA sequence having 100 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 5’ end has complementary nucleotides for part T6_Trigger_1_phi29 (BBa_K4438601) to bind. The middle region has an ssDNA sense T7 promoter sequence and the 3’ end has an ssDNA sense broccoli light-up aptamer sequence. | + | T6_Target_1 is a single-stranded DNA sequence having 100 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 5’ end has complementary nucleotides for part T6_Trigger_1_phi29 (<partinfo>BBa_K4438601<\partinfo>) to bind. The middle region has an ssDNA sense T7 promoter sequence and the 3’ end has an ssDNA sense broccoli light-up aptamer sequence. |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This part along with T6_Trigger_1_phi29 (BBa_K4438601) and Aptamer_T6 (BBa_K4438600) can be used for sensing different concentrations of testosterone. | + | This part along with T6_Trigger_1_phi29 (<partinfo>BBa_K4438601<\partinfo>) and Aptamer_T6 (<partinfo>BBa_K4438600<\partinfo>) can be used for sensing different concentrations of testosterone. |
| − | + | [[File:T--IISER-Tirupati_India--T6_1.png]] | |
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Revision as of 12:23, 12 October 2022
T6_Target_1
T6_Target_1 is a single-stranded DNA sequence having 100 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 5’ end has complementary nucleotides for part T6_Trigger_1_phi29 (No part name specified with partinfo tag.
References
Skouridou, V., Jauset-Rubio, M., Ballester, P., Bashammakh, A. S., El-Shahawi, M. S., Alyoubi, A. O., & O’Sullivan, C. K. (2017). Selection and characterization of DNA aptamers against the steroid testosterone. Microchimica Acta, 184(6), 1631-1639.