Difference between revisions of "Part:BBa K4509669:Design"
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===References=== | ===References=== | ||
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+ | 1)[https://www.sciencedirect.com/science/article/abs/pii/0022283686901713 Freudl, R., MacIntyre, S., Degen, M., & Henning, U. (1986). Cell surface exposure of the outer membrane protein OmpA of Escherichia coli K-12. Journal of molecular biology, 188(3), 491-494.] | ||
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+ | 2)[https://journals.asm.org/doi/full/10.1128/IAI.00096-09 Gaddy, J. A., Tomaras, A. P., & Actis, L. A. (2009). The Acinetobacter baumannii 19606 OmpA protein plays a role in biofilm formation on abiotic surfaces and in the interaction of this pathogen with eukaryotic cells. Infection and immunity, 77(8), 3150-3160.] | ||
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+ | 3)[https://onlinelibrary.wiley.com/doi/abs/10.1002/elsc.201700200 3.Kleiner‐Grote, G. R., Risse, J. M., & Friehs, K. (2018). Secretion of recombinant proteins from E. coli. Engineering in Life Sciences, 18(8), 532-550.] | ||
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+ | 4)[https://www.jbc.org/article/S0021-9258(18)45908-X/fulltext Sugawara, E., & Nikaido, H. (1992). Pore-forming activity of OmpA protein of Escherichia coli. Journal of Biological Chemistry, 267(4), 2507-2511.] | ||
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+ | 5)[https://www.sciencedirect.com/science/article/abs/pii/S0006291X0296657X 5.Wang, Y. (2002). The function of OmpA in Escherichia coli. Biochemical and biophysical research communications, 292(2), 396-401.] |
Latest revision as of 12:22, 12 October 2022
Cell surface aphA with Cd2+ sensing promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1012
Design Notes
The metal sensitive promotor was chosen to ensure that acid phosphatase is expressed only in presence of cadmium. This would prevent the chassis demise due to high burden.
Source
<To be added>