Difference between revisions of "Part:BBa K4298001:Design"
 
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
 
+
The original gRNA sequence on pyEVR was replaced by TM8.3 target sequence by PCR as homologous arm.
  
 
===Source===
 
===Source===
−
Assembly with K4298002;K4298000.
+
Assembly with K4298002; K4298000.
 +
K4298002 and K4298000 were from the plasmid pyEVR.

Latest revision as of 12:17, 12 October 2022


yEvolvr-TM8.3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 6414
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 6414
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4656
    Illegal BamHI site found at 6674
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 6414
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 6414
    Illegal NgoMIV site found at 1566
    Illegal NgoMIV site found at 5567
    Illegal AgeI site found at 5064
    Illegal AgeI site found at 6654
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 205
    Illegal SapI.rc site found at 1647

Design Notes

The original gRNA sequence on pyEVR was replaced by TM8.3 target sequence by PCR as homologous arm.

Source

Assembly with K4298002; K4298000. K4298002 and K4298000 were from the plasmid pyEVR.