Difference between revisions of "Part:BBa K4169025"
Ruixuan Zhu (Talk | contribs) |
Ruixuan Zhu (Talk | contribs) |
||
Line 9: | Line 9: | ||
<!-- --> | <!-- --> | ||
+ | ===Sequence and Features=== | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4169025 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4169025 SequenceAndFeatures</partinfo> | ||
Line 14: | Line 15: | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
− | + | ||
<partinfo>BBa_K4169025 parameters</partinfo> | <partinfo>BBa_K4169025 parameters</partinfo> | ||
<!-- --> | <!-- --> |
Revision as of 12:12, 12 October 2022
The sequence between two reverse loxP sites can be flipped after accepting Cre's action
It is a 34bp DNA sequence consisting of two 13bp inverted repeats and an asymmetric 8bp spacer.The Cre recognizes the inverted repeats at each end of the loxP site and binds to form a dimer, which then binds to a dimer at the other loxP site to form a tetramer. If two loxP sites are located on the same DNA strand and in opposite directions, Cre recombinase mediates sequence flip between loxP.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]