Difference between revisions of "Part:BBa K4169025"
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===Functional Parameters===
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===Sequence and Features=== -->
 
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<partinfo>BBa_K4169025 parameters</partinfo>
 
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Revision as of 12:12, 12 October 2022


The sequence between two reverse loxP sites can be flipped after accepting Cre's action

It is a 34bp DNA sequence consisting of two 13bp inverted repeats and an asymmetric 8bp spacer.The Cre recognizes the inverted repeats at each end of the loxP site and binds to form a dimer, which then binds to a dimer at the other loxP site to form a tetramer. If two loxP sites are located on the same DNA strand and in opposite directions, Cre recombinase mediates sequence flip between loxP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]