Difference between revisions of "Part:BBa K4169025"
Ruixuan Zhu (Talk | contribs) |
|||
| Line 3: | Line 3: | ||
<partinfo>BBa_K4169025 short</partinfo> | <partinfo>BBa_K4169025 short</partinfo> | ||
| − | It is a 34bp DNA sequence consisting of two 13bp inverted repeats and an asymmetric 8bp spacer.The Cre recognizes the inverted repeats at each end of the loxP site and binds to form a dimer, which then binds to a dimer at the other loxP site to form a tetramer. If two loxP sites are located on the same DNA strand and in opposite directions, Cre recombinase mediates sequence | + | It is a 34bp DNA sequence consisting of two 13bp inverted repeats and an asymmetric 8bp spacer.The Cre recognizes the inverted repeats at each end of the loxP site and binds to form a dimer, which then binds to a dimer at the other loxP site to form a tetramer. If two loxP sites are located on the same DNA strand and in opposite directions, Cre recombinase mediates sequence flip between loxP. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 12:08, 12 October 2022
The sequence between two reverse loxP sites can be flipped after accepting Cre's action
It is a 34bp DNA sequence consisting of two 13bp inverted repeats and an asymmetric 8bp spacer.The Cre recognizes the inverted repeats at each end of the loxP site and binds to form a dimer, which then binds to a dimer at the other loxP site to form a tetramer. If two loxP sites are located on the same DNA strand and in opposite directions, Cre recombinase mediates sequence flip between loxP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]