Difference between revisions of "Part:BBa K4229062"
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<partinfo>BBa_K4229062 short</partinfo>
 
<partinfo>BBa_K4229062 short</partinfo>
  
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mVenus2 is with mTurqouise2(BBa_K4229064) one of our reporter genes, with which we tested the building and catching of the minimal(BBa_K4229047) and full wiffleball(BBa_K4229049) and SPD5 (BBa_K4229078). It has the spyCatcher fused on the N-terminal of the protein to make it recruitable inside those compartments. For experimental data look into Biobrick BBa_K4229066.
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mVenus2 (together with mTurqouise2,[BBa_K4229064]) was used as reporter by fusing it with a SpyCatcher, enabling recruitment by the SpyTag. We used this fusion protein to test the funcitonality of our compartments: the minimal (BBa_K4229047) and full wiffleball (BBa_K4229049), and SPD5 (BBa_K4229078). All compartmentalisation proteins have an N-terminal SpyTag. For experimental data please refer to the data listed in Biobrick BBa_K4229066.
  
  

Revision as of 11:56, 12 October 2022


mVenus2 with N-terminal spyCatcher

mVenus2 (together with mTurqouise2,[BBa_K4229064]) was used as reporter by fusing it with a SpyCatcher, enabling recruitment by the SpyTag. We used this fusion protein to test the funcitonality of our compartments: the minimal (BBa_K4229047) and full wiffleball (BBa_K4229049), and SPD5 (BBa_K4229078). All compartmentalisation proteins have an N-terminal SpyTag. For experimental data please refer to the data listed in Biobrick BBa_K4229066.


Principle of catching proteins via Spy/Snp-Catcher by T1 and their incorporation into the BMCs, illustrated as an example of incorporating mVenus in the full wiffleball



















Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 982
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 415
  • 1000
    COMPATIBLE WITH RFC[1000]