Difference between revisions of "Part:BBa K4298001"

 
 
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<partinfo>BBa_K4298001 short</partinfo>
 
<partinfo>BBa_K4298001 short</partinfo>
  
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Evolvr device, enCas9+poll5M fusion protein with SV40 NLS,guided by sgRNA and causing random mutation of target sequence in the genome
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Evolvr device, enCas9+poll5M fusion protein with SV40 NLS,guided by sgRNA and causing random mutation of target sequence in the genome.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 11:46, 12 October 2022


yEvolvr-TM8.3

Evolvr device, enCas9+poll5M fusion protein with SV40 NLS,guided by sgRNA and causing random mutation of target sequence in the genome.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 6414
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 6414
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4656
    Illegal BamHI site found at 6674
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 6414
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 6414
    Illegal NgoMIV site found at 1566
    Illegal NgoMIV site found at 5567
    Illegal AgeI site found at 5064
    Illegal AgeI site found at 6654
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 205
    Illegal SapI.rc site found at 1647