Difference between revisions of "Part:BBa K4204021:Design"
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===Source=== | ===Source=== | ||
| − | Yang et al's work [1]. | + | The protein quality control system is from Yang et al's work [1], the CDS of LysPBC5 is from Lee et al's work [2], and other components are from BBa_K653001. Assembled by BNDS_China 2022. |
===References=== | ===References=== | ||
Revision as of 11:45, 12 October 2022
IPTG induced LysPBC5 with ProQC system
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 350
Illegal NheI site found at 1468 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1045
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1097
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The toehold switch would be at the 5' end of the mRNA with a corresponding trigger at the 3' end of the mRNA. This synthetic protein quality control system is believed to have no limitations regarding its use in other bacteria, and this system can be applied to improve the quality of recombinant protein production and also efficiencies of metabolic pathways.
Source
The protein quality control system is from Yang et al's work [1], the CDS of LysPBC5 is from Lee et al's work [2], and other components are from BBa_K653001. Assembled by BNDS_China 2022.
References
[1] Yang J, Han YH, Im J, Seo SW. Synthetic protein quality control to enhance full-length translation in bacteria. Nat Chem Biol. 2021 Apr;17(4):421-427. doi: 10.1038/s41589-021-00736-3. Epub 2021 Feb 4. PMID: 33542534.
[2] Lee, K. O., Kong, M., Kim, I., Bai, J., Cha, S., Kim, B., Ryu, K.-S., Ryu, S., & Suh, J.-Y. (2019). Structural basis for cell-wall recognition by bacteriophage PBC5 endolysin. Structure, 27(9). https://doi.org/10.1016/j.str.2019.07.001