Difference between revisions of "Part:BBa K4438606"
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<partinfo>BBa_K4438606 short</partinfo> | <partinfo>BBa_K4438606 short</partinfo> | ||
| − | T6_Target_3 is a single-stranded DNA sequence having 84 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 3’ end has complementary nucleotides for part T6_Trigger__phi29 (BBa_K4438601) to bind. The middle region has ssDNA antisense T7 promoter sequence and the 5’ end has ssDNA antisense (cDNA) broccoli light-up aptamer sequence. | + | T6_Target_3 is a single-stranded DNA sequence having 84 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 3’ end has complementary nucleotides for part T6_Trigger__phi29 (<partinfo>BBa_K4438601</partinfo>) to bind. The middle region has ssDNA antisense T7 promoter sequence and the 5’ end has ssDNA antisense (cDNA) broccoli light-up aptamer sequence. |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This part along with T6_Trigger_3_phi29 (BBa_K4438605) and Aptamer_T6 (BBa_K4438600) [1] can be used for sensing different concentrations of testosterone. | + | This part along with T6_Trigger_3_phi29 (<partinfo>BBa_K4438605</partinfo>) and Aptamer_T6 (<partinfo>BBa_K4438600</partinfo>) [1] can be used for sensing different concentrations of testosterone. |
| − | + | [[File:T--IISER-Tirupati_India--T6_3.png]] | |
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Latest revision as of 11:43, 12 October 2022
T6_Target_3
T6_Target_3 is a single-stranded DNA sequence having 84 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 3’ end has complementary nucleotides for part T6_Trigger__phi29 (BBa_K4438601) to bind. The middle region has ssDNA antisense T7 promoter sequence and the 5’ end has ssDNA antisense (cDNA) broccoli light-up aptamer sequence.
Usage and Biology
This part along with T6_Trigger_3_phi29 (BBa_K4438605) and Aptamer_T6 (BBa_K4438600) [1] can be used for sensing different concentrations of testosterone.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 76
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 76
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 76
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 76
- 1000COMPATIBLE WITH RFC[1000]
References
Skouridou, V., Jauset-Rubio, M., Ballester, P., Bashammakh, A. S., El-Shahawi, M. S., Alyoubi, A. O., & O’Sullivan, C. K. (2017). Selection and characterization of DNA aptamers against the steroid testosterone. Microchimica Acta, 184(6), 1631-1639.