Difference between revisions of "Part:BBa K200015:Design"
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===Source=== | ===Source=== | ||
| − | Synthesised from the genomic sequence. | + | Synthesised from the genomic sequence of Streptococcus pneumoniae Hungary19A-6. |
===References=== | ===References=== | ||
Latest revision as of 09:39, 14 October 2009
Coding region for the DpnII restriction enzyme.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
NOTE: Unlike other restriction enzymes that show Star Activity at high pH 8.0, DpnII has Star Activity above approximately pH 6.5. The effect of pH on Star Activity of DpnII is very extreme, showing one-thousand-fold more Star Activity at pH 7.5 than at pH 6.0.
Source
Synthesised from the genomic sequence of Streptococcus pneumoniae Hungary19A-6.