Difference between revisions of "Part:BBa K4162029"
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===Introduction=== | ===Introduction=== | ||
| − | [[File:T--Fudan--logo-Rester-rectangle.jpg|100px|right| | + | [[File:T--Fudan--logo-Rester-rectangle.jpg|100px|right|2022 Fudan]] |
Coding sequence of BCMO and gene ''ybbO'' separated by hammerhead ribozyme. Gene ''ybbO'' is tagged with tdMCP and EGFP. | Coding sequence of BCMO and gene ''ybbO'' separated by hammerhead ribozyme. Gene ''ybbO'' is tagged with tdMCP and EGFP. | ||
Revision as of 11:39, 12 October 2022
ribozyme + T7_RBS + BCMO + ribozyme + T7_RBS + ybbO-tdMCP-EGFP
Introduction
Coding sequence of BCMO and gene ybbO separated by hammerhead ribozyme. Gene ybbO is tagged with tdMCP and EGFP.
We use this biobrick to verify the expression ybbO in the presence of BCMO. Please check our measurement page for details.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 2521
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 2521
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 2521
Illegal AgeI site found at 1018
Illegal AgeI site found at 1611 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2101
Illegal BsaI site found at 2455