Difference between revisions of "Part:BBa K4162029"

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Coding sequence of BCMO and gene ''ybbO'' separated by hammerhead ribozyme. Gene ''ybbO'' is tagged with tdMCP and EGFP.
 
Coding sequence of BCMO and gene ''ybbO'' separated by hammerhead ribozyme. Gene ''ybbO'' is tagged with tdMCP and EGFP.
  
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We use this biobrick to verify the expression ybbO, which could not be induced and seen by SDS-PAGE. Please check our [https://2022.igem.wiki/fudan/measurement#fluorescence-microscopy-to-confirm-the-successful-expression-of-membrane-proteins measurement] page for details.
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We use this biobrick to verify the expression ybbO in the presence of BCMO. Please check our [https://2022.igem.wiki/fudan/measurement#fluorescence-microscopy-to-confirm-the-successful-expression-of-membrane-proteins measurement] page for details.
  
 
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Revision as of 11:37, 12 October 2022


ribozyme + T7_RBS + BCMO + ribozyme + T7_RBS + ybbO-tdMCP-EGFP

Introduction

2021 Fudan

Coding sequence of BCMO and gene ybbO separated by hammerhead ribozyme. Gene ybbO is tagged with tdMCP and EGFP.

We use this biobrick to verify the expression ybbO in the presence of BCMO. Please check our measurement page for details.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2521
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2521
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2521
    Illegal AgeI site found at 1018
    Illegal AgeI site found at 1611
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2101
    Illegal BsaI site found at 2455