Difference between revisions of "Part:BBa K4204019:Design"
Jianfei Song (Talk | contribs) (→References) |
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===Source=== | ===Source=== | ||
| − | The protein quality control system is from Yang et al's work [1]. Other components are from BBa_K653003.Assembled by BNDS_China 2022. | + | The protein quality control system is from Yang et al's work [1]. Other components are from BBa_K653003. Assembled by BNDS_China 2022. |
===References=== | ===References=== | ||
[1] Yang, J., Han, Y.H., Im, J. et al. Synthetic protein quality control to enhance full-length translation in bacteria. Nat Chem Biol 17, 421–427 (2021). https://doi.org/10.1038/s41589-021-00736-3 | [1] Yang, J., Han, Y.H., Im, J. et al. Synthetic protein quality control to enhance full-length translation in bacteria. Nat Chem Biol 17, 421–427 (2021). https://doi.org/10.1038/s41589-021-00736-3 | ||
Revision as of 11:32, 12 October 2022
IPTG induced GFP improved with ProQC system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 316
Illegal NheI site found at 1116 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 978
Design Notes
The GFP could be replaced by any protein in interest.
Source
The protein quality control system is from Yang et al's work [1]. Other components are from BBa_K653003. Assembled by BNDS_China 2022.
References
[1] Yang, J., Han, Y.H., Im, J. et al. Synthetic protein quality control to enhance full-length translation in bacteria. Nat Chem Biol 17, 421–427 (2021). https://doi.org/10.1038/s41589-021-00736-3