Difference between revisions of "Part:BBa K4477012:Design"
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===Source=== | ===Source=== | ||
− | 1. https://www.jacc.org/doi/full/10.1016/j.jacc.2011.07.017 | + | 1. https://www.jacc.org/doi/full/10.1016/j.jacc.2011.07.017 <br> |
2. https://pubmed.ncbi.nlm.nih.gov/14644097/ | 2. https://pubmed.ncbi.nlm.nih.gov/14644097/ | ||
===References=== | ===References=== | ||
3. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3230644/ | 3. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3230644/ |
Revision as of 11:23, 12 October 2022
IK17 (anti-oxLDL) scFv - complete expression cassette
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 576
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Amino acid sequences were reverse transcribed and codon optimized for expression in E. coli B (the parent strain of SHuffle). In addition, further codon optimization was used to remove illegal restriction sites from the construct.
Source
1. https://www.jacc.org/doi/full/10.1016/j.jacc.2011.07.017
2. https://pubmed.ncbi.nlm.nih.gov/14644097/