Difference between revisions of "Part:BBa K4119026"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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<partinfo>BBa_K4119026 parameters</partinfo> | <partinfo>BBa_K4119026 parameters</partinfo> | ||
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| + | <meta http-equiv="X-UA-Compatible" content="IE=edge"> | ||
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| + | <title>Document</title> | ||
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| + | <p style="font-size: 180%; font-weight: bold;">Results:</p> | ||
| + | <p>After modification, both experimental groups had lower expression levels than the positive control group, and we found that the pMTL82151-PvgbF3-Bs2 experimental group did not achieve the purpose of the transformation. However，the pMTL82151-PvgbF7-Bs2 experimental group showed aerobic inhibition and microoxide expression characteristics.</p> | ||
| + | <p><img src="https://static.igem.wiki/teams/4119/wiki/jt-files/h2/img13.png" width="80%" height="80%"></p> | ||
| + | <div align="center"> | ||
| + | <strong>Figure. Expression effect of PvgbF3-bs2, PvgbF7-bs2 and Pvgb at different oxygen concentrations</strong> | ||
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| + | <span class='h3bb'>Sequence and Features</span> | ||
| + | <partinfo>BBa_K4119026 SequenceAndFeatures</partinfo> | ||
Latest revision as of 11:21, 12 October 2022
Pvgb-separate FNR binding site from -35->Bs2->Cpa fdx terminator
PvgbF3/PvgbF7-Bs2-Cpa fdx terminator
Results:
After modification, both experimental groups had lower expression levels than the positive control group, and we found that the pMTL82151-PvgbF3-Bs2 experimental group did not achieve the purpose of the transformation. However，the pMTL82151-PvgbF7-Bs2 experimental group showed aerobic inhibition and microoxide expression characteristics.
Figure. Expression effect of PvgbF3-bs2, PvgbF7-bs2 and Pvgb at different oxygen concentrations
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 357
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 357
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 357
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 357
- 1000COMPATIBLE WITH RFC[1000]