Difference between revisions of "Part:BBa K4438115"
 
Line 3: Line 3:
 
<partinfo>BBa_K4438115 short</partinfo>
 
<partinfo>BBa_K4438115 short</partinfo>
  
−
FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. FASTmiR-27a-D3, after transcription, binds to miR-27a-5p, the transcription product of BBa_K4438502. The binding of these to RNAs will lead to the revelation of a binding site for DFHBI, a fluorophore, where it gets trapped and shows fluorescence.
+
FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. FASTmiR-27a-D3, after transcription, binds to miR-27a-5p, the transcription product of <partinfo>BBa_K4438152</partinfo>. The binding of these to RNAs will lead to the revelation of a binding site for DFHBI, a fluorophore, where it gets trapped and shows fluorescence.
  
  
  
 
===Usage and Biology===
 
===Usage and Biology===
−
This part can be used for the detection of miR-27a-5p, which proves to be a biomarker for many diseases. The ON and OFF structure for the sensor was generated as given:
+
This part can be used for the detection of miR-27a-5p, which proves to be a biomarker for many diseases. The OFF(A) and ON(B) structure for the sensor was generated as given:
−
[[File:https://static.igem.wiki/teams/4438/wiki/model/aptamer-design-collage/mirna-27a5p-3.png|centre]]
+
[[File:T--IISER-Tirupati_India--miRNA_27a5p_3.png]]
  
 
<!-- -->
 
<!-- -->

Latest revision as of 11:19, 12 October 2022


FASTmiR-27a-D3

FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. FASTmiR-27a-D3, after transcription, binds to miR-27a-5p, the transcription product of BBa_K4438152. The binding of these to RNAs will lead to the revelation of a binding site for DFHBI, a fluorophore, where it gets trapped and shows fluorescence.


Usage and Biology

This part can be used for the detection of miR-27a-5p, which proves to be a biomarker for many diseases. The OFF(A) and ON(B) structure for the sensor was generated as given: T--IISER-Tirupati India--miRNA 27a5p 3.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 49
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 49
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 49
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 49
  • 1000
    COMPATIBLE WITH RFC[1000]


Notes

The split position of the complementary sequence in the sensor is TGCTCACAAG….CAGCTAAGCCCT

References

Huang, K., Doyle, F., Wurz, Z. E., Tenenbaum, S. A., Hammond, R. K., Caplan, J. L., & Meyers, B. C. (2017). FASTmiR: an RNA-based sensor for in vitro quantification and live-cell localization of small RNAs. Nucleic acids research, 45(14), e130-e130