Difference between revisions of "Part:BBa K4501022"

 
Line 3: Line 3:
 
<partinfo>BBa_K4501022 short</partinfo>
 
<partinfo>BBa_K4501022 short</partinfo>
  
−
This plasmid backbone, based on the pJUMP29-1A KanR TypeIIS Level 1 pUC backbone from the distribution kit, has been upgraded to incorporate the 5' and 3' MSCV modules required for a lentiviral transfer plasmid of 2nd and 3rd generation. The MSCV modules are incorporated on the secondary restriction sites for BbsI and PaqCI located at the sides of the main assembly site. The plasmid is ready for a BsmbI-based cloning assembly and subsequent direct use as transfer plasmid.
+
Disclaimer. This is a plasmid backbone and therefore the compatibility labels are not applicable.
 +
This plasmid backbone, based on the pJUMP29-1A KanR TypeIIS Level 1 pUC backbone from the distribution kit, has been upgraded to incorporate the 5' and 3' modules required for a lentiviral transfer plasmid of 2nd and 3rd generation. The modules (BBa_K4501005 and BBa_K4501006) are incorporated on the secondary restriction sites for BbsI and PaqCI located at the sides of the main assembly site. The plasmid is ready for a BsmbI-based cloning assembly and subsequent direct use as transfer plasmid.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
 +
This plasmid allows to skip several steps of cloning into a lentiviral transfer plasmid, providing versatility and amenability; by assembling the construction directly to a transfer plasmid! Main assembly (BsaI or BsmBI based cloning) could be performed and the plasmid is ready to be used as a transfer plasmid right away together with either 2nd or 3rd packaging plasmids.
  
 
<!-- -->
 
<!-- -->

Revision as of 11:19, 12 October 2022


pJUMP lentiviral transfer plasmid

Disclaimer. This is a plasmid backbone and therefore the compatibility labels are not applicable. This plasmid backbone, based on the pJUMP29-1A KanR TypeIIS Level 1 pUC backbone from the distribution kit, has been upgraded to incorporate the 5' and 3' modules required for a lentiviral transfer plasmid of 2nd and 3rd generation. The modules (BBa_K4501005 and BBa_K4501006) are incorporated on the secondary restriction sites for BbsI and PaqCI located at the sides of the main assembly site. The plasmid is ready for a BsmbI-based cloning assembly and subsequent direct use as transfer plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 5061
    Illegal suffix found at 5105
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5061
    Illegal SpeI site found at 5106
    Illegal PstI site found at 5120
    Illegal NotI site found at 5067
    Illegal NotI site found at 5113
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5061
    Illegal BglII site found at 33
    Illegal BglII site found at 74
    Illegal BglII site found at 3682
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 5061
    Illegal suffix found at 5106
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 5061
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5076
    Illegal SpeI site found at 5106
    Illegal PstI site found at 5120
    Illegal NgoMIV site found at 1895
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 2693
    Illegal SapI.rc site found at 4194