Difference between revisions of "Part:BBa K4438111"
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<partinfo>BBa_K4438111 short</partinfo> | <partinfo>BBa_K4438111 short</partinfo> | ||
| − | FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. FASTmiR-30c-D2, after transcription, binds to miR-30c, the transcription product of | + | FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. FASTmiR-30c-D2, after transcription, binds to miR-30c, the transcription product of <partinfo>BBa_K4438153<partinfo>. The binding of these to RNAs will lead to the revelation of a binding site for DFHBI, a fluorophore, where it gets trapped and shows fluorescence. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This part can be used for the detection of miR-30c, which proves to be a biomarker for many diseases. The | + | This part can be used for the detection of miR-30c, which proves to be a biomarker for many diseases. The OFF(A) and ON(B) structure for the sensor was generated as given: |
| − | + | [[File:T--IISER-Tirupati_India--miRNA_30c1_2.png]] | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 11:09, 12 October 2022
FASTmiR-30c-D2
FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. FASTmiR-30c-D2, after transcription, binds to miR-30c, the transcription product of No part name specified with partinfo tag.
References
Huang, K., Doyle, F., Wurz, Z. E., Tenenbaum, S. A., Hammond, R. K., Caplan, J. L., & Meyers, B. C. (2017). FASTmiR: an RNA-based sensor for in vitro quantification and live-cell localization of small RNAs. Nucleic acids research, 45(14), e130-e130