Difference between revisions of "Part:BBa K4169024"
Ruixuan Zhu (Talk | contribs) |
Ruixuan Zhu (Talk | contribs) |
||
| Line 5: | Line 5: | ||
Two loxP sites are on the same DNA strand and in opposite directions. And after expressing, it'll produce Cyclic recombinase which mediates sequence flip between loxPs. | Two loxP sites are on the same DNA strand and in opposite directions. And after expressing, it'll produce Cyclic recombinase which mediates sequence flip between loxPs. | ||
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here --> |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | When we express the Cre which recognizes the reverse repeat sequence at both ends of the loxP site and binds to form a dimer, which then binds to a dimer at another loxP site to form a tetramer.This allows us to have our engineered bacteria flip the gene between the two reverse loxP sites. | |
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 11:09, 12 October 2022
produce Cre enzyme
Two loxP sites are on the same DNA strand and in opposite directions. And after expressing, it'll produce Cyclic recombinase which mediates sequence flip between loxPs.
Usage and Biology
When we express the Cre which recognizes the reverse repeat sequence at both ends of the loxP site and binds to form a dimer, which then binds to a dimer at another loxP site to form a tetramer.This allows us to have our engineered bacteria flip the gene between the two reverse loxP sites. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 356
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 42
- 1000COMPATIBLE WITH RFC[1000]