Difference between revisions of "Part:BBa K4164016"
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We connected ddRFPA1 and ddRFPB1 by a flexible linker(3*GGGGS) to verify the feasicility of ddRFPA1 and ddRFPB1. Following overnight incubation at 37℃, we can see the bright red fluorescence (Fig.1). In this case, we chose ddRFPA1 and ddRFPB1 as our report device. | We connected ddRFPA1 and ddRFPB1 by a flexible linker(3*GGGGS) to verify the feasicility of ddRFPA1 and ddRFPB1. Following overnight incubation at 37℃, we can see the bright red fluorescence (Fig.1). In this case, we chose ddRFPA1 and ddRFPB1 as our report device. | ||
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<p style="text-align: center;"><img src="https://static.igem.wiki/teams/4164/wiki/part-registry/part-004005016998ddrfpplate.png"with="1000" height="" width="500" height=""/></p> | <p style="text-align: center;"><img src="https://static.igem.wiki/teams/4164/wiki/part-registry/part-004005016998ddrfpplate.png"with="1000" height="" width="500" height=""/></p> | ||
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<p style="text-align: center!important;"><b>Fig.1 Fluorescence image of <em> E. coli</em> expressing ddRFPA1-ddRFPB1 and control.</b></p> | <p style="text-align: center!important;"><b>Fig.1 Fluorescence image of <em> E. coli</em> expressing ddRFPA1-ddRFPB1 and control.</b></p> | ||
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Latest revision as of 11:03, 12 October 2022
Inductive expression of ddRFPA1-ddRFPB1
This composite part was constructed to analyze the function of ddRFP and the intensity of the T7 lac promoter.
The composite part can be directly imported into plasmid and express ddRFPA1-ddRFPB1 induced with IPTG.
We connected ddRFPA1 and ddRFPB1 by a flexible linker(3*GGGGS) to verify the feasicility of ddRFPA1 and ddRFPB1. Following overnight incubation at 37℃, we can see the bright red fluorescence (Fig.1). In this case, we chose ddRFPA1 and ddRFPB1 as our report device.
Fig.1 Fluorescence image of E. coli expressing ddRFPA1-ddRFPB1 and control.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1271
Illegal NheI site found at 1539 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 830
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]