Difference between revisions of "Part:BBa K4501002:Experience"
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We demonstrated the downregulation of myc by transiently transfecting (by electroporation) these shRNA in a expression cassette on Ramos and HEK293T cell lines. qPCR from total RNA extraction shows as high as almost 2-fold decrease in myc expression when treatment with shRNA. | We demonstrated the downregulation of myc by transiently transfecting (by electroporation) these shRNA in a expression cassette on Ramos and HEK293T cell lines. qPCR from total RNA extraction shows as high as almost 2-fold decrease in myc expression when treatment with shRNA. | ||
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Figure 1. Results of qPCR for Ramos and HEK293T cell line. | Figure 1. Results of qPCR for Ramos and HEK293T cell line. | ||
Latest revision as of 10:53, 12 October 2022
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Applications of BBa_K4501002
We demonstrated the downregulation of myc by transiently transfecting (by electroporation) these shRNA in a expression cassette on Ramos and HEK293T cell lines. qPCR from total RNA extraction shows as high as almost 2-fold decrease in myc expression when treatment with shRNA.
Figure 1. Results of qPCR for Ramos and HEK293T cell line.
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