Difference between revisions of "Part:BBa K4417010"

 
 
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<partinfo>BBa_K4417010 short</partinfo>
 
<partinfo>BBa_K4417010 short</partinfo>
  
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<h1>Description</h1>
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This part codes for the ureEFG operon from ''Sporosarcina pasteurii'', enabling the expression of the three accessory subunits of urease enzymes.
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[[File:Zjy27.png|600px|thumb|center|'''Figure 1:''' ureABC operon from ''Sporosarcina pasteurii''. ]]
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<h1>Usage and Biology</h1>
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ureEFG are urease (accessory) proteins, responsible for the activation of the ureABC proenzyme. ureEFG is responsible for the transport and assembly of the nickel II center.
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[[File:Zjy26.png|600px|thumb|center|'''Figure 2:''' Urease assembly and maturation process (Veaudor T, 2019). Chaperones are ureDEFG proteins. ureD will assemble with ureF. Ni binding with ureE helps the ureG dimer recruitment. Final chaperons will involve in GTP hydrolysis for urease maturation. ]]
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<h1>Reference</h1>
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1. Veaudor T, Cassier-Chauvat C, Chauvat F. Genomics of Urea Transport and Catabolism in Cyanobacteria: Biotechnological Implications. Front Microbiol. 2019 Sep 4;10:2052. doi: 10.3389/fmicb.2019.02052. PMID: 31551986; PMCID: PMC6737895.
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2. Masepohl, B et al. “Urea utilization in the phototrophic bacterium Rhodobacter capsulatus is regulated by the transcriptional activator NtrC.” Journal of bacteriology vol. 183,2 (2001): 637-43. doi:10.1128/JB.183.2.637-643.2001
  
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===Usage and Biology===
 
  
 
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Latest revision as of 10:50, 12 October 2022


ureEFG

Description

This part codes for the ureEFG operon from Sporosarcina pasteurii, enabling the expression of the three accessory subunits of urease enzymes.

Figure 1: ureABC operon from Sporosarcina pasteurii.

Usage and Biology

ureEFG are urease (accessory) proteins, responsible for the activation of the ureABC proenzyme. ureEFG is responsible for the transport and assembly of the nickel II center.

Figure 2: Urease assembly and maturation process (Veaudor T, 2019). Chaperones are ureDEFG proteins. ureD will assemble with ureF. Ni binding with ureE helps the ureG dimer recruitment. Final chaperons will involve in GTP hydrolysis for urease maturation.


Reference

1. Veaudor T, Cassier-Chauvat C, Chauvat F. Genomics of Urea Transport and Catabolism in Cyanobacteria: Biotechnological Implications. Front Microbiol. 2019 Sep 4;10:2052. doi: 10.3389/fmicb.2019.02052. PMID: 31551986; PMCID: PMC6737895.

2. Masepohl, B et al. “Urea utilization in the phototrophic bacterium Rhodobacter capsulatus is regulated by the transcriptional activator NtrC.” Journal of bacteriology vol. 183,2 (2001): 637-43. doi:10.1128/JB.183.2.637-643.2001


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 877
    Illegal XhoI site found at 1631
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]