Difference between revisions of "Part:BBa K4244054"

 
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<partinfo>BBa_K4244054 short</partinfo>
 
<partinfo>BBa_K4244054 short</partinfo>
  
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This plasmid contains one component out of three of the three-step inducible system. Here the behaviour of the system with different lengths of the P1 spacer can be tested by measuring the uvGFP fluorescence. This construct consists of uvGFP coding sequence with a dSpyCas9 target site in between the promoters and coding sequence of this protein. Additionally, an introduction template for sgRNA spacers was introduced, this template contains two type IIS restriction sides, this way different length P1 spacers can be easily introduced and tested.
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This plasmid contains one component out of three of the three-step inducible system. Here, the behaviour of the system with different lengths of the P1 spacer can be tested by measuring the uvGFP fluorescence. This construct consists of uvGFP coding sequence with a dSpyCas9 target site in between the promoters and coding sequence of this protein. Additionally, an introduction template for sgRNA spacers was introduced. This template contains two type IIS restriction sides, this way different length P1 spacers can be easily introduced and tested.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 10:48, 12 October 2022


Three-step inducible system with uvGFP

This plasmid contains one component out of three of the three-step inducible system. Here, the behaviour of the system with different lengths of the P1 spacer can be tested by measuring the uvGFP fluorescence. This construct consists of uvGFP coding sequence with a dSpyCas9 target site in between the promoters and coding sequence of this protein. Additionally, an introduction template for sgRNA spacers was introduced. This template contains two type IIS restriction sides, this way different length P1 spacers can be easily introduced and tested.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 151
    Illegal XbaI site found at 157
    Illegal XbaI site found at 248
    Illegal SpeI site found at 30
    Illegal PstI site found at 169
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 151
    Illegal NheI site found at 7
    Illegal SpeI site found at 30
    Illegal PstI site found at 169
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 151
    Illegal BglII site found at 181
    Illegal BamHI site found at 791
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 151
    Illegal XbaI site found at 157
    Illegal XbaI site found at 248
    Illegal SpeI site found at 30
    Illegal PstI site found at 169
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 151
    Illegal XbaI site found at 157
    Illegal XbaI site found at 248
    Illegal SpeI site found at 30
    Illegal PstI site found at 169
  • 1000
    COMPATIBLE WITH RFC[1000]