Difference between revisions of "Part:BBa K4244055"

 
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<partinfo>BBa_K4244055 short</partinfo>
 
<partinfo>BBa_K4244055 short</partinfo>
  
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This plasmid contains one component out of three of the three-step inducible system. Here the behaviour of the system with different lengths of the t5 spacer can be tested by measuring the BFP fluorescence. This construct consists of BFP coding sequence with a dSpyCas9 target site in between the promoters and coding sequence of this protein. Additionally, an introduction template for sgRNA spacers was introduced, this template contains two type IIS restriction sides, this way different length t5 spacers can be easily introduced and tested.
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This plasmid contains one component out of three of the three-step inducible system. Here, the behaviour of the system with different lengths of the t5 spacer can be tested by measuring the BFP fluorescence. This construct consists of BFP coding sequence with a dSpyCas9 target site in between the promoters and coding sequence of this protein. Additionally, an introduction template for sgRNA spacers was introduced. This template contains two type IIS restriction sides, this way different length t5 spacers can be easily introduced and tested.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 10:47, 12 October 2022


Three-step inducible system with BFP

This plasmid contains one component out of three of the three-step inducible system. Here, the behaviour of the system with different lengths of the t5 spacer can be tested by measuring the BFP fluorescence. This construct consists of BFP coding sequence with a dSpyCas9 target site in between the promoters and coding sequence of this protein. Additionally, an introduction template for sgRNA spacers was introduced. This template contains two type IIS restriction sides, this way different length t5 spacers can be easily introduced and tested.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 151
    Illegal XbaI site found at 157
    Illegal SpeI site found at 30
    Illegal PstI site found at 169
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 151
    Illegal NheI site found at 7
    Illegal SpeI site found at 30
    Illegal PstI site found at 169
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 151
    Illegal BglII site found at 181
    Illegal BamHI site found at 849
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 151
    Illegal XbaI site found at 157
    Illegal SpeI site found at 30
    Illegal PstI site found at 169
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 151
    Illegal XbaI site found at 157
    Illegal SpeI site found at 30
    Illegal PstI site found at 169
  • 1000
    COMPATIBLE WITH RFC[1000]