Difference between revisions of "Part:BBa K4417009"

 
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<h1>Description</h1>
  
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This part codes for the ureABC operon from ''Sporosarcina pasteurii'', enabling the expression of the three subunits of urease enzymes.
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===Usage and Biology===
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[[File:Zjy25.png|600px|thumb|center|'''Figure 1:''' ureABC operon from ''Sporosarcina pasteurii'']]
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<h1>Usage and Biology</h1>
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* Urease (EC 3.5.1.5) is an enzyme that catalyses urea hydrolysis, forming carbon dioxide and ammonia. In biomineralization, carbonic anhydrase acts as a hydrator, catalysing the reaction of carbon dioxide (CO<sub>2</sub>) with water into carbonic acid, which spontaneously decays into carbonate ions.
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* ureABC are urease structural genes.
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* Most bacterial urease has a trimer structure (ureABC)<sub>3</sub> of two small (ureA and ureB) and one large (ureC) subunit.
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[[File:Zjy26.png|600px|thumb|center|'''Figure 2:''' Urease assembly and maturation process (Veaudor T, 2019). Urease catalytic subunits (ureA, ureB, and ureC) will assemble automatically and start carbamylation, resulting in inactive apo-urease ure(ABC)3. With the presence of accessory protein, Ni will bind with apo-urease ure(ABC)<sub>3</sub> turning into active holo-urease.]]
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<h1>Reference</h1>
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1. Veaudor T, Cassier-Chauvat C, Chauvat F. Genomics of Urea Transport and Catabolism in Cyanobacteria: Biotechnological Implications. Front Microbiol. 2019 Sep 4;10:2052. doi: 10.3389/fmicb.2019.02052. PMID: 31551986; PMCID: PMC6737895.
  
 
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Revision as of 10:44, 12 October 2022


ureABC

Description

This part codes for the ureABC operon from Sporosarcina pasteurii, enabling the expression of the three subunits of urease enzymes.

Figure 1: ureABC operon from Sporosarcina pasteurii


Usage and Biology

  • Urease (EC 3.5.1.5) is an enzyme that catalyses urea hydrolysis, forming carbon dioxide and ammonia. In biomineralization, carbonic anhydrase acts as a hydrator, catalysing the reaction of carbon dioxide (CO2) with water into carbonic acid, which spontaneously decays into carbonate ions.
  • ureABC are urease structural genes.
  • Most bacterial urease has a trimer structure (ureABC)3 of two small (ureA and ureB) and one large (ureC) subunit.
Figure 2: Urease assembly and maturation process (Veaudor T, 2019). Urease catalytic subunits (ureA, ureB, and ureC) will assemble automatically and start carbamylation, resulting in inactive apo-urease ure(ABC)3. With the presence of accessory protein, Ni will bind with apo-urease ure(ABC)3 turning into active holo-urease.

Reference

1. Veaudor T, Cassier-Chauvat C, Chauvat F. Genomics of Urea Transport and Catabolism in Cyanobacteria: Biotechnological Implications. Front Microbiol. 2019 Sep 4;10:2052. doi: 10.3389/fmicb.2019.02052. PMID: 31551986; PMCID: PMC6737895.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]