Difference between revisions of "Part:BBa K4238004"

 
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<partinfo>BBa_K4238004 short</partinfo>
 
<partinfo>BBa_K4238004 short</partinfo>
  
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When transformed into yeast together with BBa_K4238003, this Part causes the yeast to produce red fluorescence under blue light.
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The C120-trucCYC1promoter causes dimeric EL222 to bind to the C120 sequence under blue light, allowing transcription of the downstream sequence.
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The expression can be quantified with the fluorescence of mCherry. A kill switch (BBa_K4238005) with the human Bax gene instead of mCherry was also created.
  
 
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Revision as of 10:40, 12 October 2022


C120-trucCYC1p+mCherry+ADH1t

When transformed into yeast together with BBa_K4238003, this Part causes the yeast to produce red fluorescence under blue light.

The C120-trucCYC1promoter causes dimeric EL222 to bind to the C120 sequence under blue light, allowing transcription of the downstream sequence. The expression can be quantified with the fluorescence of mCherry. A kill switch (BBa_K4238005) with the human Bax gene instead of mCherry was also created.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 104
    Illegal SpeI site found at 79
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 86
    Illegal SpeI site found at 79
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 104
    Illegal SpeI site found at 79
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 104
    Illegal SpeI site found at 79
  • 1000
    COMPATIBLE WITH RFC[1000]