Difference between revisions of "Part:BBa K266001"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K266001 short</partinfo>
 
<partinfo>BBa_K266001 short</partinfo>
  
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[[Part:BBa_J23100|BBa_J23100]] constitutive promoter directs the expression of tetracycline repressor  ([[Part:BBa_C0040|TetR]]). TetR binds to [[Part:BBa_R0040|pTet]] regulatory region resulting in a ''negative control'' of the production of LuxR. The whole system acts as an ''Inverter'' of Tet system controlling LuxR expression.
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[[Part:BBa_J23100|J23100]] constitutive promoter directs the expression of tetracycline repressor  ([[Part:BBa_C0040|TetR]]). TetR binds to [[Part:BBa_R0040|pTet]] regulatory region resulting in a ''negative control'' of the production of LuxR. The whole system acts as an ''Inverter'' of Tet system controlling LuxR expression.
 
TetR repression is inhibited by the addition of tetracycline or its analog, [http://openwetware.org/wiki/ATc aTc].
 
TetR repression is inhibited by the addition of tetracycline or its analog, [http://openwetware.org/wiki/ATc aTc].
  

Latest revision as of 19:32, 13 October 2009

Inverter TetR -> LuxR

J23100 constitutive promoter directs the expression of tetracycline repressor (TetR). TetR binds to pTet regulatory region resulting in a negative control of the production of LuxR. The whole system acts as an Inverter of Tet system controlling LuxR expression. TetR repression is inhibited by the addition of tetracycline or its analog, [http://openwetware.org/wiki/ATc aTc].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]