Difference between revisions of "Part:BBa K4165256"
(→‎WetLab Results)
Line 51: Line 51:
 
                 COH TD28Rev, showing that the interaction between Tau aggregates with GST COH WWW is better than that of Tau  
 
                 COH TD28Rev, showing that the interaction between Tau aggregates with GST COH WWW is better than that of Tau  
 
                 aggregates with GST COH TD28Rev as the concentration of elution of Tau aggregates with GST COH WWW is more  
 
                 aggregates with GST COH TD28Rev as the concentration of elution of Tau aggregates with GST COH WWW is more  
−
                 than that of Tau aggregates with GST COH TD28Rev
+
                 than that of Tau aggregates with GST COH TD28Rev, illustrating that WWW could be a better candidate for
 +
                binding to the Tau aggregates
  
  

Revision as of 10:30, 12 October 2022


GST-Coh2-G4S-WWW

Long description

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85

WetLab Results

Transformation of GST COH WWW in BL-21 using pGS-21a vector 

                                    Figure 1. Transformed plate of GST COH (L) WWW + pGS-21a

Transformation of GST COH WWW in DH-5 alpha using pJET vector

                                   Figure 2. Transformed plate of GST COH (L) WWW + pJET

Comparison between chemical lysis and sonication for GST COH WWW 

                  Figure 3. This graph shows a significant difference between chemical lysis and sonication for GST COH 
                     WWW, after we had the results we optimized our protocol to use chemical lysis for GST COH WWW

SDS PAGE for induced and non induced samples of GST COH WWW 

               Figure 4. This figure shows the comparison between induced and non induced samples of GST COH WWW, where well 
                 no.1 is the induced sample while well no.5 is the non induced sample showing that our protein is induced 
                 effectively owing to our right choice of IPTC, time interval and concentration

Pull down assay of His Trim21 (L) against GST COH WWW and GST COH TD28Rev 

              Figure 5. This graph shows the comparison of pull down assay between His trim21(L) DOC with GST COH WWW and 
               GST COH TD28Rev, showing that the interaction between His Trim21 (L) DOC and GST COH WWW is better than that 
               of His Trim21 (L) DOC and GST COH TD28Rev as the concentration of the elution of His Trim21 (L) DOC with GST 
               COH WWW is more than that of His Trim21 (L) DOC with GST COH TD28Rev

Pull down assay of Tau aggregates against GST COH WWW and GST COH TD28Rev 

              Figure 6. This graph shows the comparison of pull down assay between Tau aggregates with GST COH WWW and GST 
               COH TD28Rev, showing that the interaction between Tau aggregates with GST COH WWW is better than that of Tau 
               aggregates with GST COH TD28Rev as the concentration of elution of Tau aggregates with GST COH WWW is more 
               than that of Tau aggregates with GST COH TD28Rev, illustrating that WWW could be a better candidate for 
               binding to the Tau aggregates