Difference between revisions of "Part:BBa K4229036"

Latest revision as of 10:30, 12 October 2022

T3 protein from the full wiffleball of the BMC

The T3 proteins and T2-proteins(BBa_K4229035) assemble and form the second pore structure (together with T1 in the full wiffleball[BBa_K4229048/BBa_K4229049]).

A:Showing the original HO-Shell found in Haliangium orchaceum. B: showing the full wiffleball made with H-/T1-/T2-/T3-protein. C: minimalwiffleball made just our of the H- and T1-protein.

The plasmid with this protein was kindly sent to us by the Kerfeld Group after we found this very interesting paper:

H. Kirst and C. A. Kerfeld, “Bacterial microcompartments: Catalysis-enhancing metabolic modules for next-generation metabolic and biomedical engineering,” BMC Biol., vol. 17, no. 1, pp. 1–11, 2019, DOI: 10.1186/s12915-019-0691-z.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 566
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 186
Illegal NgoMIV site found at 542
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4229036 short</partinfo>
-The T3 proteins builds together with the T2-protein(BBa_K4229035) the second pore structure beside T1 in the full wiffleball(BBa_K4229048/BBa_K4229049).
+The T3 proteins and T2-proteins(BBa_K4229035) assemble and form the second pore structure (together with T1 in the full wiffleball[BBa_K4229048/BBa_K4229049]).
 [[File:BMCs3.jpg|600px|thumb|left|A:Showing the original HO-Shell found in Haliangium orchaceum. B: showing the full wiffleball made with H-/T1-/T2-/T3-protein. C: minimalwiffleball made just our of the H- and T1-protein. ]]
@@ Line 51: / Line 51: @@
-The plasmid with this protein was sent kindly send to us by the Kerfeld Group after we found this very interesting paper:
+The plasmid with this protein was kindly sent to us by the Kerfeld Group after we found this very interesting paper:
 H. Kirst and C. A. Kerfeld, “Bacterial microcompartments: Catalysis-enhancing metabolic modules for next-generation metabolic and biomedical engineering,” BMC Biol., vol. 17, no. 1, pp. 1–11, 2019, DOI: 10.1186/s12915-019-0691-z.