Difference between revisions of "Part:BBa K4229029"

Latest revision as of 10:27, 12 October 2022

BMC-H Protein

The H-protein assembles the basic shell of the wiffelballs (minimal-wiffelball: BBa_K4229047/ full-wiffelball: BBa_K4229049) that we used as compartmentalisation system. It was desinged after the HO-shell found in Haliangium orchaceum.

A:Shows the original HO-Shell found in Haliangium orchaceum. B: Shows the full wiffleball made with H-/T1-/T2-/T3-protein. C: minimal wiffleball made just of the H- and T1-protein.

The plasmid containing this protein was kindly sent to us by the Kerfeld Group after we found this very interesting paper:

H. Kirst and C. A. Kerfeld, “Bacterial microcompartments: Catalysis-enhancing metabolic modules for next-generation metabolic and biomedical engineering,” BMC Biol., vol. 17, no. 1, pp. 1–11, 2019, DOI: 10.1186/s12915-019-0691-z.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4229029 short</partinfo>
-The H-protein buildes the basic shell in the wiffelballs (minimal-wiffelball: BBa_K4229047/ full-wiffelball: BBa_K4229049) we used in our project as one of our compartments. It was desinged after the HO-shell found in Haliangium orchaceum.
+The H-protein assembles the basic shell of the wiffelballs (minimal-wiffelball: BBa_K4229047/ full-wiffelball: BBa_K4229049) that we used as compartmentalisation system. It was desinged after the HO-shell found in <i>Haliangium orchaceum</i>.
-[[File:BMCs3.jpg|600px|thumb|left|A:Showing the original HO-Shell found in Haliangium orchaceum. B: showing the full wiffleball made with H-/T1-/T2-/T3-protein. C: minimal wiffleball made just our of the H- and T1-protein. ]]
+[[File:BMCs3.jpg|600px|thumb|left| A:Shows the original HO-Shell found in  <i>Haliangium orchaceum</i>. B: Shows the full wiffleball made with H-/T1-/T2-/T3-protein. C: minimal wiffleball made just of the H- and T1-protein. ]]
@@ Line 50: / Line 50: @@
-The plasmid containing this protein was sent kindly send to us by the Kerfeld Group after we found this very interesting paper:
+The plasmid containing this protein was kindly sent to us by the Kerfeld Group after we found this very interesting paper:
 H. Kirst and C. A. Kerfeld, “Bacterial microcompartments: Catalysis-enhancing metabolic modules for next-generation metabolic and biomedical engineering,” BMC Biol., vol. 17, no. 1, pp. 1–11, 2019, DOI: 10.1186/s12915-019-0691-z.