Difference between revisions of "Part:BBa K4275041"

Revision as of 10:24, 12 October 2022

pT7-RBS-CipA1B2C-tT7

Strong promoter and terminator are used for high production.The protein produced has the ability to interact with 2 enzymatic subunits that are fused with a type I dockerin because the primary scaffoldin contains 2 type I cohesin domain. The cohesin-dockerin interaction fixes the subunits onto the primary scaffold allowing the enzymes to work synergetically to perform a cascade of catalytical reactions. One CBM ensures the cellulose or PET in the surrounding are attracted to the domains and allows them to be digested.

Usage and Biology

The protein scaffold of the mimic primary scaffoldin component contains 2 type I cohesin domain that interacts with the type I dockerin domain fused on other fusion protein to fix them onto the scaffold as a complex, the CBM domain on the protein attracts PET and cellulose in the surrounding, generating close proximity between the fixed enzyme on the scaffold and the substrate in the case of cellulose and PET degradation, for the catalytical reactions to happen faster. The primary scaffold can also be assembled to the secondary scaffold as it contains a type II dockerin domain to construct larger cellulosome-like complexes. And the paired strong promoter and terminator grants efficient production of the protein.

Sequence and Features

References

1. Anandharaj, Marimuthu et al. "Constructing A Yeast To Express The Largest Cellulosome Complex On The Cell Surface". Proceedings Of The National Academy Of Sciences, vol 117, no. 5, 2020, pp. 2385-2394. Proceedings Of The National Academy Of Sciences, https://doi.org/10.1073/pnas.1916529117.