Difference between revisions of "Part:BBa K174011"
(New page: __NOTOC__ <partinfo>BBa_K174011 short</partinfo> Spo0A is the master regulator of sporulation in ''B. subtilis''. When it is phosphorylated, it activates the expression of more than 100 g...) |
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By artifically synthesising KinA during growth, spoulation can be triggered in a multicomponent phosphorelay in which KinA phosphorylates Spo0F, Spo0F~P in turn phosphorylates Spo0B, and finally Spo0B~P phosphorylates Spo0A. (2) | By artifically synthesising KinA during growth, spoulation can be triggered in a multicomponent phosphorelay in which KinA phosphorylates Spo0F, Spo0F~P in turn phosphorylates Spo0B, and finally Spo0B~P phosphorylates Spo0A. (2) | ||
| − | Spo0A~ P initiates sporulation first by activating low-threshold genes and then by gradually increasing and activating high-threshold genes. Hence the multicomponent phosphorelay’s elements, KinA, Spo0F and spo0B, gradually increases spo0A and its activity, and initiates the sporulation(2). | + | Expressing Spo0A itself alone in the cell would not initiate the sporulation. Spo0A~ P initiates sporulation, first by activating low-threshold genes, and then by gradually increasing and activating high-threshold genes. Hence the multicomponent phosphorelay’s elements, KinA, Spo0F and spo0B, gradually increases spo0A and its activity, and initiates the sporulation. (2). |
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Revision as of 18:17, 13 October 2009
IPTG inducable kinA Bacillus sporulation trigger
Spo0A is the master regulator of sporulation in B. subtilis. When it is phosphorylated, it activates the expression of more than 100 genes(1). It has been shown that, activation of spo0A is sufficient to trigger sporulation.
By artifically synthesising KinA during growth, spoulation can be triggered in a multicomponent phosphorelay in which KinA phosphorylates Spo0F, Spo0F~P in turn phosphorylates Spo0B, and finally Spo0B~P phosphorylates Spo0A. (2)
Expressing Spo0A itself alone in the cell would not initiate the sporulation. Spo0A~ P initiates sporulation, first by activating low-threshold genes, and then by gradually increasing and activating high-threshold genes. Hence the multicomponent phosphorelay’s elements, KinA, Spo0F and spo0B, gradually increases spo0A and its activity, and initiates the sporulation. (2).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- Veening, J.-W., W. K. Smits, et al. (2008). "Bistability, Epigenetics, and Bet-Hedging in Bacteria." Annual Review of Microbiology 62(1): 193-210.
- Fujita, M. and R. Losick (2005). "Evidence that entry into sporulation in Bacillus subtilis is governed by a gradual increase in the level and activity of the master regulator Spo0A." Genes & Development 19(18): 2236-2244.