Difference between revisions of "Part:BBa K4181016"
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And that sequence is used to produce tyrosinase which reacts with levodopa to produce melanin, which is used to protect the DNA. | And that sequence is used to produce tyrosinase which reacts with levodopa to produce melanin, which is used to protect the DNA. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | <p>The coding sequence of tyrosinase was present in the genome of Bacillus polymorpha, but we were unsuccessful in borrowing the Bacillus polymorpha strain, so we still chose to synthesize tyrosinase directly by subscription. The sequence was synthesized by removing the start codon and adding the linker sequence at the 5' end and adding a 6*His tag before the stop codon at the 3' end for subsequent detection. </p> | |
| + | [[File:File:2022-SJTU-Biox-Shanghai-10.png|500px|center]]<br> | ||
| + | <p align="center">'''Figure 1.''' Image of tyrosinase-encoding gene by PCR followed by electrophoresis. The picture indicates a positive result.<br></p> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 10:23, 12 October 2022
Tyrosinase protein DNA sequence
And that sequence is used to produce tyrosinase which reacts with levodopa to produce melanin, which is used to protect the DNA.
Usage and Biology
The coding sequence of tyrosinase was present in the genome of Bacillus polymorpha, but we were unsuccessful in borrowing the Bacillus polymorpha strain, so we still chose to synthesize tyrosinase directly by subscription. The sequence was synthesized by removing the start codon and adding the linker sequence at the 5' end and adding a 6*His tag before the stop codon at the 3' end for subsequent detection.
Figure 1. Image of tyrosinase-encoding gene by PCR followed by electrophoresis. The picture indicates a positive result.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 298
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 298
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 303
Illegal XhoI site found at 491 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 298
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 298
- 1000COMPATIBLE WITH RFC[1000]