Difference between revisions of "Part:BBa K4275039"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | <i>Kluyveromyces marxianus</i> mating factor alpha is an secretion signal in <i>Kluyveromyces marxianus</i>, which encodes for an alpha mating factor domain fused with target protein MtCDH-t. The signal peptide in alpha mating factor domain would guide the protein into RER and Golgi apparatus for | + | <i>Kluyveromyces marxianus</i> mating factor alpha is an secretion signal in <i>Kluyveromyces marxianus</i>, which encodes for an alpha mating factor domain fused with target protein MtCDH-t. The signal peptide in alpha mating factor domain would guide the protein into RER and Golgi apparatus for the secretion of MtCDH-t. |
MtCDH-t is considered a cellulase booster with strong reductive nature, enabling it to act as an electron donor to another cellulase booster TaLPMO-t during crystalline cellulose degradation. The free cellulase booster is converted into cellulosomal mode via the fusion of a type I dockerin to its C terminal. Functioning synergistically with four other cellulase and booster enzymes, MtCDH-t contributes towards effective biodegradation of cellulose components. | MtCDH-t is considered a cellulase booster with strong reductive nature, enabling it to act as an electron donor to another cellulase booster TaLPMO-t during crystalline cellulose degradation. The free cellulase booster is converted into cellulosomal mode via the fusion of a type I dockerin to its C terminal. Functioning synergistically with four other cellulase and booster enzymes, MtCDH-t contributes towards effective biodegradation of cellulose components. | ||
Revision as of 10:21, 12 October 2022
pLAC4-KmarxMFα-MtCDH-t-tTDH1
The composite part is constructed from Kluyveromyces marxianus mating factor alpha (BBa_K4275000) and cellulase booster MtCDH-t fused with type I dockerin (BBa_K4275005).
The fusion of Kluyveromyces marxianus mating factor alpha (BBa_K4275000) ensures effective secretion of MtCDH-t protein by the host yeast, thus avoiding lysing the yeast cell to extract the designated cellulase booster.
Usage and Biology
Kluyveromyces marxianus mating factor alpha is an secretion signal in Kluyveromyces marxianus, which encodes for an alpha mating factor domain fused with target protein MtCDH-t. The signal peptide in alpha mating factor domain would guide the protein into RER and Golgi apparatus for the secretion of MtCDH-t.
MtCDH-t is considered a cellulase booster with strong reductive nature, enabling it to act as an electron donor to another cellulase booster TaLPMO-t during crystalline cellulose degradation. The free cellulase booster is converted into cellulosomal mode via the fusion of a type I dockerin to its C terminal. Functioning synergistically with four other cellulase and booster enzymes, MtCDH-t contributes towards effective biodegradation of cellulose components.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 331
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 331
Illegal NheI site found at 89 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 331
Illegal BglII site found at 3987
Illegal XhoI site found at 1252
Illegal XhoI site found at 4150 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 331
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 331
Illegal NgoMIV site found at 2788
Illegal AgeI site found at 1639
Illegal AgeI site found at 2026
Illegal AgeI site found at 2152
Illegal AgeI site found at 2701 - 1000COMPATIBLE WITH RFC[1000]