Difference between revisions of "Part:BBa K174010"

 
 
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<partinfo>BBa_K174010 short</partinfo>
 
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Spo0A is the master regulator of sporulation in ''B. subtilis''. When it is phosphorylated, it activates the expression of more than 100 genes. It has been shown that, activation of spo0A is sufficient to trigger sporulation.
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Spo0A is the master regulator of sporulation in ''B. subtilis''. When it is phosphorylated, it activates the expression of more than 100 genes(1). It has been shown that, activation of spo0A is sufficient to trigger sporulation.
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By artifically synthesising KinA during growth, spoulation can be triggered in a multicomponent phosphorelay in which KinA phosphorylates Spo0F, Spo0F~P in turn phosphorylates Spo0B, and finally Spo0B~P phosphorylates Spo0A.
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By artifically synthesising KinA during growth, spoulation can be triggered in a multicomponent phosphorelay in which KinA phosphorylates Spo0F, Spo0F~P in turn phosphorylates Spo0B, and finally Spo0B~P phosphorylates Spo0A. (2)
  
 
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<partinfo>BBa_K174010 parameters</partinfo>
 
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===References===
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#Veening, J.-W., W. K. Smits, et al. (2008). "Bistability, Epigenetics, and Bet-Hedging in Bacteria." Annual Review of Microbiology 62(1): 193-210.
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#Fujita, M. and R. Losick (2005). "Evidence that entry into sporulation in Bacillus subtilis is governed by a gradual increase in the level and activity of the master regulator Spo0A." Genes & Development 19(18): 2236-2244.

Latest revision as of 17:38, 13 October 2009

KinA

Spo0A is the master regulator of sporulation in B. subtilis. When it is phosphorylated, it activates the expression of more than 100 genes(1). It has been shown that, activation of spo0A is sufficient to trigger sporulation.

By artifically synthesising KinA during growth, spoulation can be triggered in a multicomponent phosphorelay in which KinA phosphorylates Spo0F, Spo0F~P in turn phosphorylates Spo0B, and finally Spo0B~P phosphorylates Spo0A. (2)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. Veening, J.-W., W. K. Smits, et al. (2008). "Bistability, Epigenetics, and Bet-Hedging in Bacteria." Annual Review of Microbiology 62(1): 193-210.
  2. Fujita, M. and R. Losick (2005). "Evidence that entry into sporulation in Bacillus subtilis is governed by a gradual increase in the level and activity of the master regulator Spo0A." Genes & Development 19(18): 2236-2244.