Difference between revisions of "Part:BBa K4432301"

 
 
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This part is a transcription unit of the PANTR1 Trigger N°1 ([[Part:BBa_K4432201|BBa_K4432201]]) for sequence-based detection of PANTR1, a long non coding (lnc) RNA overexpressed in different types of cancer cells.
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The transcription is controlled by the T7 promoter ([[Part:BBa_K2150031|BBa_K2150031]]) and the strong SBa_000587 synthetic terminator ([[Part:BBa_K3453000|BBa_K3453000]]).
  
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===Usage and Biology===
 
===Usage and Biology===
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A toehold switch [1] (Figure 1) is an RNA-based device is composed of several parts which are essential for the efficient functioning of our biosensor :
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- a Trigger Binding Site (TBS) which is a sequence complementary to the RNA target to be detected
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- a Ribosome Binding Site (RBS) which allows the start of the translation only when the toehold switch is unfolded
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- a start codon AUG which constitutes the start of the translation
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- a linker which constitutes a bridge between the Start codon and our gene of interest
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- a repressed gene of interest that is translated into a protein only when the hairpin is unfolded and the RBS exposed
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The functioning comes as follows :
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The binding between the RNA biomarker and the toehold switch unfolds the hairpin structure. The RBS becomes accessible now so that the translation can take place, and the initially repressed gene of interest can then be translated into a protein.
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[[File: T--Evry_Paris-Saclay--PANTR1_Toehold.jpg|600px]]
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Figure 1. Principle of the toehold switch (inspired from iGEM Evry Paris-Saclay 2020).
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This part is a transcription unit of [[Part:BBa_K4432201|BBa_K4432201]] which is a trigger for the PANTR1 Toehold Switch N°1 ([[Part:BBa_K4432001|BBa_K4432001]]).
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This part proved to be functional: the transcribed PANTR1 RNA fragment was able to bind to the corresponding toehold switches and a readable output was generated.
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Full results are available on the [[Part:BBa_K4432101|BBa_K4432101]] page in the registry.
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===References===
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[1] Green AA, Silver PA, Collins JJ, Yin P. Toehold switches: de-novo-designed regulators of gene expression. Cell (2014) 159, 925-939.
  
 
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Latest revision as of 10:12, 12 October 2022


PANTR1 Toehold Switch Trigger 01 Transcriptional Unit

This part is a transcription unit of the PANTR1 Trigger N°1 (BBa_K4432201) for sequence-based detection of PANTR1, a long non coding (lnc) RNA overexpressed in different types of cancer cells.

The transcription is controlled by the T7 promoter (BBa_K2150031) and the strong SBa_000587 synthetic terminator (BBa_K3453000).

Usage and Biology

A toehold switch [1] (Figure 1) is an RNA-based device is composed of several parts which are essential for the efficient functioning of our biosensor :

- a Trigger Binding Site (TBS) which is a sequence complementary to the RNA target to be detected

- a Ribosome Binding Site (RBS) which allows the start of the translation only when the toehold switch is unfolded

- a start codon AUG which constitutes the start of the translation

- a linker which constitutes a bridge between the Start codon and our gene of interest

- a repressed gene of interest that is translated into a protein only when the hairpin is unfolded and the RBS exposed

The functioning comes as follows :

The binding between the RNA biomarker and the toehold switch unfolds the hairpin structure. The RBS becomes accessible now so that the translation can take place, and the initially repressed gene of interest can then be translated into a protein.

T--Evry Paris-Saclay--PANTR1 Toehold.jpg

Figure 1. Principle of the toehold switch (inspired from iGEM Evry Paris-Saclay 2020).

This part is a transcription unit of BBa_K4432201 which is a trigger for the PANTR1 Toehold Switch N°1 (BBa_K4432001).

This part proved to be functional: the transcribed PANTR1 RNA fragment was able to bind to the corresponding toehold switches and a readable output was generated.

Full results are available on the BBa_K4432101 page in the registry.

References

[1] Green AA, Silver PA, Collins JJ, Yin P. Toehold switches: de-novo-designed regulators of gene expression. Cell (2014) 159, 925-939.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]