Difference between revisions of "Part:BBa K4488005"
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<b>Usage</b> Has been used in a composite part with Neae-intimin for expression in E. coli. See part BBa_K4488027 | <b>Usage</b> Has been used in a composite part with Neae-intimin for expression in E. coli. See part BBa_K4488027 | ||
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[[File:modelling-sf-h.gif|centre|thumb|700px| Modelling of binding of Nanobody H (3OGO-H) to sfGFP made using python package LightDock]] | [[File:modelling-sf-h.gif|centre|thumb|700px| Modelling of binding of Nanobody H (3OGO-H) to sfGFP made using python package LightDock]] | ||
Latest revision as of 10:11, 12 October 2022
Anti-GFP Nanobody/sdAb
Nanobody binding GFP. Optimised for expression in Escherichia coli. Binding to a fluorescent protein allows for fluorescent tagging and testing of constructs. PDB: 3OGO-H. Esp3I golden gate sites flank either end of this part. Analogous to BBa_K929102
Usage Has been used in a composite part with Neae-intimin for expression in E. coli. See part BBa_K4488027
References: Twair, A., Al-Okla, S., Zarkawi, M., & Abbady, A. Q. (2014). Characterization of camel nanobodies specific for superfolder GFP fusion proteins. Molecular biology reports, 41(10), 6887–6898. https://doi.org/10.1007/s11033-014-3575-x
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]