Difference between revisions of "Part:BBa K4501005"

 
Line 3: Line 3:
 
<partinfo>BBa_K4501005 short</partinfo>
 
<partinfo>BBa_K4501005 short</partinfo>
  
−
Encodes for the 5' LTR and the psi sequences, used on the 5' terminus of lentiviral transfer plasmids. The 5' LTR acts as an RNA polymerase II promoter, initiating the transcription of the transgene. The psi sequence is a RNA target site for packaging by the nucleocapsid.
+
This 5' module codifies for the main 5' upstream components needed in a lentiviral transfer plasmid. Spceifically, it encodes for the T3 and RSV promoters, 5' LTR, psi sequence, RRE and gp41 peptide. The 5' LTR acts as an RNA polymerase II promoter, initiating the transcription of the transgene. The psi sequence is a RNA target site for packaging by the nucleocapsid.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:10, 12 October 2022


MSCV 5' module

This 5' module codifies for the main 5' upstream components needed in a lentiviral transfer plasmid. Spceifically, it encodes for the T3 and RSV promoters, 5' LTR, psi sequence, RRE and gp41 peptide. The 5' LTR acts as an RNA polymerase II promoter, initiating the transcription of the transgene. The psi sequence is a RNA target site for packaging by the nucleocapsid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 268
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 780