Difference between revisions of "Part:BBa K4488003"

Revision as of 10:09, 12 October 2022

Anti-GFP Nanobody/sdAb

Nanobody binding GFP. Optimised for expression in Escherichia coli. Binding to a fluorescent protein allows for fluorescent tagging and testing of constructs. GenBank ID: AIF28006

Usage Has been used in a composite part with Neae-intimin for expression in E. coli. See part BBa_K4488029

modelling of Nanobody 6 (AIF28006) to sfGFP made using python package Lightdock

References: Twair, A., Al-Okla, S., Zarkawi, M., & Abbady, A. Q. (2014). Characterization of camel nanobodies specific for superfolder GFP fusion proteins. Molecular biology reports, 41(10), 6887–6898. https://doi.org/10.1007/s11033-014-3575-x

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 Nanobody binding GFP. Optimised for expression in Escherichia coli. Binding to a fluorescent protein allows for fluorescent tagging and testing of constructs. GenBank ID: AIF28006
-Usage Has been used in a composite part with Neae-intimin for expression in E. coli. See part BBa_K4488029
+<b>Usage</b> Has been used in a composite part with Neae-intimin for expression in E. coli. See part BBa_K4488029
 [[File:modelling-sf-6.gif|centre|thumb|700px| modelling of Nanobody 6 (AIF28006) to sfGFP made using python package Lightdock]]