Difference between revisions of "Part:BBa K4488002"
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Nanobody binding sfGFP. Optimised for expression in Escherichia coli. Binding to a fluorescent protein allows for fluorescent tagging and testing of constructs. GenBank ID:AIF28003 | Nanobody binding sfGFP. Optimised for expression in Escherichia coli. Binding to a fluorescent protein allows for fluorescent tagging and testing of constructs. GenBank ID:AIF28003 | ||
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+ | <b>Usage</b> Has been used in a composite part with Neae-intimin for expression in E. coli. See part BBa_K4488028 | ||
+ | [[File:modelling-sf-3.gif|centre|thumb|700px| Modelling of binding of Nanobody 3 (AIF28003) to sfGFP]] | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Revision as of 09:56, 12 October 2022
Anti-GFP Nanobody/sdAb
Nanobody binding sfGFP. Optimised for expression in Escherichia coli. Binding to a fluorescent protein allows for fluorescent tagging and testing of constructs. GenBank ID:AIF28003
Usage Has been used in a composite part with Neae-intimin for expression in E. coli. See part BBa_K4488028
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]