Difference between revisions of "Part:BBa K4165255"

Revision as of 09:54, 12 October 2022

GST-Coh2-G4S- TD28REV

Long description

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 689
Illegal SapI.rc site found at 85

WetLab Results

Transformation of GST COH (L) TD28 Rev in BL-21 using pGS-21a vector

                                Figure 1. Transformed plate of GST COH (L) TD28 Rev + pGS-21a

Transformation of GST COH (L) TD21 Rev in DH-5 alpha using pJET vector

                               Figure 2. Transformed plate of GST COH (L) TD28 Rev + pJET

Comparison between chemical lysis and sonication for GST COH TD28 Rev

                      Figure 3. this graph shows a difference between chemical lysis and sonication for GST COH TD28 
                       Rev, after we had the results we optimized our protocol to use chemical lysis for GST COH (L) 
                       TD28 Rev

SDS PAGE of induced and non induced samples of GST COH TD28 Rev

                     Figure 4. This figure shows the comparison between the induced and non induced samples of GST COH 
                      TD28Rev, where well no.2 is the induced sample of GST COH TD28 Rev while well no.6 is the non induced 
                      sample of GST COH TD28Rev showing that our protein is induced effectively owing to our right choice of 
                      IPTG, time interval and concentration

Pull down assay His Trim (L) DOC against GST COH WWW and GST COH TD28Rev

                     Figure 5. This graph shows the comparison of pull down assay between His Trim21 (L) DOC with GST COH 
                      WWW and GST COH TD28Rev showing that the interaction between His Trim21 (L) DOC and GST COH WWW is 
                      better than that of His Trim21 (L) DOC with GAT COH TD28Rev as the concentration of elution of His 
                      Trim21 (L) DOC with GST COH WWW is more than that of His trim21 (L) DOC with GST COH TD28Rev

@@ Line 27: / Line 27: @@
 <p><img src="https://static.igem.wiki/teams/4165/wiki/data-analysis/sonication-or-chemical/sonication-or-chemical/coh-td28rev.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 </html>
-Figure 3. this graph shows a difference between chemical lysis and sonication for GST COH TD28 Rev, after we had the results we optimized our protocol to use chemical lysis for GST COH (L) TD28 Rev
+                       Figure 3. this graph shows a difference between chemical lysis and sonication for GST COH TD28
+                        Rev, after we had the results we optimized our protocol to use chemical lysis for GST COH (L)
+                        TD28 Rev
 <p style=" font-weight: bold; font-size:14px;"> SDS PAGE of induced and non induced samples of GST COH TD28 Rev </p>
 <html>
 <p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/sds-of-gst-td28rev-and-www.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 </html>
-Figure 4. This figure shows the comparison between the induced and non induced samples of GST COH TD28Rev, where well no.2 is the induced sample of GST COH TD28 Rev while well no.6 is the non induced sample of GST COH TD28Rev showing that our protein is induced effectively owing to our right choice of IPTG, time interval and concentration
+                      Figure 4. This figure shows the comparison between the induced and non induced samples of GST COH
+                       TD28Rev, where well no.2 is the induced sample of GST COH TD28 Rev while well no.6 is the non induced
+                       sample of GST COH TD28Rev showing that our protein is induced effectively owing to our right choice of
+                       IPTG, time interval and concentration
+<p style=" font-weight: bold; font-size:14px;"> Pull down assay His Trim (L) DOC against GST COH WWW and GST COH TD28Rev </p>
+<html>
+<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/pull-down-trim-doc-vs-www-and-td28rev.jpg" style="margin-left:200px;" alt="" width="500" /></p>
+</html>
+                      Figure 5. This graph shows the comparison of pull down assay between His Trim21 (L) DOC with GST COH
+                       WWW and GST COH TD28Rev showing that the interaction between His Trim21 (L) DOC and GST COH WWW is
+                       better than that of His Trim21 (L) DOC with GAT COH TD28Rev as the concentration of elution of His
+                       Trim21 (L) DOC with GST COH WWW is more than that of His trim21 (L) DOC with GST COH TD28Rev
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 ===Functional Parameters===
 <partinfo>BBa_K4165255 parameters</partinfo>
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