Difference between revisions of "Part:BBa K4488005"
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Nanobody binding sfGFP. Optimised for expression in Escherichia coli. Binding to a fluorescent protein allows for fluorescent tagging and testing of constructs. PDB: 3OGO-H. Esp3I golden gate sites flank either end of this part. | Nanobody binding sfGFP. Optimised for expression in Escherichia coli. Binding to a fluorescent protein allows for fluorescent tagging and testing of constructs. PDB: 3OGO-H. Esp3I golden gate sites flank either end of this part. | ||
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| + | <b>Usage:</b> | ||
| + | This part has been used in a composite part with Neae-Intimin for expression in TOP10 E.coli. See part BBa_K4488027 | ||
| + | [[File:modelling-sf-h.gif|centre|thumb|700px| modelling of Nanobody h (3OGOH) to sfGFP]] | ||
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Revision as of 09:51, 12 October 2022
Anti-GFP Nanobody/sdAb
Nanobody binding sfGFP. Optimised for expression in Escherichia coli. Binding to a fluorescent protein allows for fluorescent tagging and testing of constructs. PDB: 3OGO-H. Esp3I golden gate sites flank either end of this part.
Usage: This part has been used in a composite part with Neae-Intimin for expression in TOP10 E.coli. See part BBa_K4488027
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]